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Index one: the i7 index column is while in the forward orientation (match the orientation Should the i7 indexes inside the sample sheet).
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Established D Index Adapters Seek advice from Index two (i5) Orientation For more info regarding how to enter i5 bases about the sample sheet in forward or reverse complement orientation.
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The sequences shown are what's entered into the ultimate sample sheet. Kit definitions contain the i5 while in the forward orientation. The i5 sequences is going to be appropriately reverse complemented and shown in the IEM interface based upon instrument/reagent sort.